| Identification |
|---|
| HMDB Protein ID
| HMDBP12571 |
| Secondary Accession Numbers
| None |
| Name
| Carbamoyl-phosphate synthase |
| Synonyms
|
- Carbamoyl phosphate synthetase
- SYN3
- CPSase
|
| Gene Name
| (GENBANK) CARBAMOYL-PHOSPHATE SYNTHASE |
| Protein Type
| Unknown |
| Biological Properties |
|---|
| General Function
| Not Available |
| Specific Function
| Catalyzes the synthesis of carbamoyl phosphate from ATP, ammonium and bicarbonate. Proceeds via a three-step mechanism, i.e. the phosphorylation of hydrogencarbonate to carboxyphosphate, a nucleophilic attack of ammonia on carboxyphosphate yielding carbamate, and the phosphorylation of carbamate forming carbamoyl phosphate. In M.smithii, the predominant archaeon in the human gut, one function of this enzyme may be to sequester ammonia, a scarce nutrient in the intestine which is the major source of nitrogen in M.smithii for the biosynthesis of nucleotides, amino acids, and many other metabolites. |
| Pathways
|
Not Available
|
| Reactions
| Not Available |
| GO Classification
|
| Molecular Function |
| manganese ion binding |
| magnesium ion binding |
| ATP binding |
| carbamoyl-phosphate synthase (ammonia) activity |
|
| Cellular Location
|
Not Available
|
| Gene Properties |
|---|
| Chromosome Location
| Not Available |
| Locus
| Not Available |
| SNPs
| Not Available |
| Gene Sequence
|
Not Available
|
| Protein Properties |
|---|
| Number of Residues
| 367 |
| Molecular Weight
| 41402.69 |
| Theoretical pI
| 4.732 |
| Pfam Domain Function
|
|
| Signals
|
Not Available
|
|
Transmembrane Regions
|
Not Available
|
| Protein Sequence
|
Not Available
|
| External Links |
|---|
| GenBank ID Protein
| Not Available |
| UniProtKB/Swiss-Prot ID
| A5UK38 |
| UniProtKB/Swiss-Prot Entry Name
| CPS_METS3 |
| PDB IDs
|
Not Available |
| GenBank Gene ID
| Not Available |
| GeneCard ID
| Not Available |
| GenAtlas ID
| Not Available |
| HGNC ID
| Not Available |
| References |
|---|
| General References
| - Samuel BS, Hansen EE, Manchester JK, Coutinho PM, Henrissat B, Fulton R, Latreille P, Kim K, Wilson RK, Gordon JI: Genomic and metabolic adaptations of Methanobrevibacter smithii to the human gut. Proc Natl Acad Sci U S A. 2007 Jun 19;104(25):10643-8. doi: 10.1073/pnas.0704189104. Epub 2007 Jun 11. [PubMed:17563350 ]
- Popa E, Perera N, Kibedi-Szabo CZ, Guy-Evans H, Evans DR, Purcarea C: The smallest active carbamoyl phosphate synthetase was identified in the human gut archaeon Methanobrevibacter smithii. J Mol Microbiol Biotechnol. 2012;22(5):287-99. doi: 10.1159/000342520. Epub 2012 Oct 27. [PubMed:23107800 ]
|
