| Identification |
|---|
| HMDB Protein ID
| HMDBP12109 |
| Secondary Accession Numbers
| None |
| Name
| Phospholipase C |
| Synonyms
|
- PLC
- Alpha-toxin
- Hemolysin
- Lecithinase
- Phosphatidylcholine cholinephosphohydrolase
|
| Gene Name
| PLC |
| Protein Type
| Unknown |
| Biological Properties |
|---|
| General Function
| Not Available |
| Specific Function
| Bacterial hemolysins are exotoxins that attack blood cell membranes and cause cell rupture. Constitutes an essential virulence factor in gas gangrene. Binds to eukaryotic membranes where it hydrolyzes both phosphatidylcholine and sphingomyelin. The diacylglycerol produced can activate both the arachidonic acid pathway, leading to modulation of the inflammatory response cascade and thrombosis, and protein kinase C, leading to activation of eukaryotic phospholipases and further membrane damage. Acts on human and mouse erythrocytes, but not on rabbit or horse erythrocytes. |
| Pathways
|
- Biosynthesis of secondary metabolites
- Glycerophospholipid metabolism
- Inositol phosphate metabolism
- Quorum sensing
|
| Reactions
| Not Available |
| GO Classification
|
| Biological Process |
| hemolysis in other organism |
| pathogenesis |
| Cellular Component |
| extracellular region |
| Molecular Function |
| phosphatidylcholine phospholipase C activity |
| toxin activity |
| calcium-dependent phospholipase C activity |
| zinc ion binding |
|
| Cellular Location
|
Not Available
|
| Gene Properties |
|---|
| Chromosome Location
| Not Available |
| Locus
| Not Available |
| SNPs
| Not Available |
| Gene Sequence
|
Not Available
|
| Protein Properties |
|---|
| Number of Residues
| 398 |
| Molecular Weight
| 45529.285 |
| Theoretical pI
| 5.898 |
| Pfam Domain Function
|
|
| Signals
|
|
|
Transmembrane Regions
|
Not Available
|
| Protein Sequence
|
Not Available
|
| External Links |
|---|
| GenBank ID Protein
| Not Available |
| UniProtKB/Swiss-Prot ID
| P0C216 |
| UniProtKB/Swiss-Prot Entry Name
| PHLC1_CLOPE |
| PDB IDs
|
|
| GenBank Gene ID
| Not Available |
| GeneCard ID
| Not Available |
| GenAtlas ID
| Not Available |
| HGNC ID
| Not Available |
| References |
|---|
| General References
| - Shimizu T, Ohtani K, Hirakawa H, Ohshima K, Yamashita A, Shiba T, Ogasawara N, Hattori M, Kuhara S, Hayashi H: Complete genome sequence of Clostridium perfringens, an anaerobic flesh-eater. Proc Natl Acad Sci U S A. 2002 Jan 22;99(2):996-1001. Epub 2002 Jan 15. [PubMed:11792842 ]
- Saint-Joanis B, Garnier T, Cole ST: Gene cloning shows the alpha-toxin of Clostridium perfringens to contain both sphingomyelinase and lecithinase activities. Mol Gen Genet. 1989 Nov;219(3):453-60. doi: 10.1007/BF00259619. [PubMed:2560137 ]
- Katayama S, Matsushita O, Minami J, Mizobuchi S, Okabe A: Comparison of the alpha-toxin genes of Clostridium perfringens type A and C strains: evidence for extragenic regulation of transcription. Infect Immun. 1993 Feb;61(2):457-63. doi: 10.1128/iai.61.2.457-463.1993. [PubMed:8423073 ]
- Tsutsui K, Minami J, Matsushita O, Katayama S, Taniguchi Y, Nakamura S, Nishioka M, Okabe A: Phylogenetic analysis of phospholipase C genes from Clostridium perfringens types A to E and Clostridium novyi. J Bacteriol. 1995 Dec;177(24):7164-70. doi: 10.1128/jb.177.24.7164-7170.1995. [PubMed:8522524 ]
- Ginter A, Williamson ED, Dessy F, Coppe P, Bullifent H, Howells A, Titball RW: Molecular variation between the alpha-toxins from the type strain (NCTC 8237) and clinical isolates of Clostridium perfringens associated with disease in man and animals. Microbiology (Reading). 1996 Jan;142 ( Pt 1):191-198. doi: 10.1099/13500872-142-1-191. [PubMed:8581165 ]
- Kameyama K, Matsushita O, Katayama S, Minami J, Maeda M, Nakamura S, Okabe A: Analysis of the phospholipase C gene of Clostridium perfringens KZ1340 isolated from Antarctic soil. Microbiol Immunol. 1996;40(4):255-63. doi: 10.1111/j.1348-0421.1996.tb03344.x. [PubMed:8709860 ]
|
