| Identification |
|---|
| HMDB Protein ID
| HMDBP11493 |
| Secondary Accession Numbers
| |
| Name
| Rho guanine nucleotide exchange factor 4 |
| Synonyms
|
- APC-stimulated guanine nucleotide exchange factor
- Asef
|
| Gene Name
| ARHGEF4 |
| Protein Type
| Unknown |
| Biological Properties |
|---|
| General Function
| Involved in Rho guanyl-nucleotide exchange factor activity |
| Specific Function
| Acts as guanine nucleotide exchange factor (GEF) for RhoA and RAC1 GTPases. Binding of APC may activate RAC1 GEF activity. The APC-ARHGEF4 complex seems to be involved in cell migration as well as in E-cadherin-mediated cell-cell adhesion |
| Pathways
|
Not Available
|
| Reactions
| Not Available |
| GO Classification
|
| Component |
| cell part |
| intracellular |
| Function |
| small gtpase regulator activity |
| ras guanyl-nucleotide exchange factor activity |
| rho guanyl-nucleotide exchange factor activity |
| enzyme regulator activity |
| nucleoside-triphosphatase regulator activity |
| gtpase regulator activity |
| guanyl-nucleotide exchange factor activity |
| Process |
| regulation of ras protein signal transduction |
| regulation of rho protein signal transduction |
| biological regulation |
| regulation of biological process |
| regulation of cellular process |
| intracellular signaling pathway |
| signaling |
| signaling pathway |
| regulation of cell communication |
| regulation of signal transduction |
| regulation of small gtpase mediated signal transduction |
|
| Cellular Location
|
- Peripheral membrane protein
- Cell projection
- Isoform 3:Cytoplasm
- Cytoplasmic side (Probable)
- ruffle membrane
|
| Gene Properties |
|---|
| Chromosome Location
| Chromosome:2 |
| Locus
| 2q22 |
| SNPs
| ARHGEF4 |
| Gene Sequence
|
>2073 bp
ATGCCCTGGGAAGAACCAGCAGGTGAGAAGCCCAGTTGCTCTCACAGTCAGAAGGCGTTC
CACATGGAGCCTGCCCAGAAGCCCTGCTTCACCACTGACATGGTGACATGGGCCCTCCTC
TGCATCTCTGCAGAGACTGTGCGTGGGGAGGCTCCTTCACAGCCTAGGGGCATCCCTCAC
CGCTCGCCCGTCAGTGTGGATGACCTGTGGCTGGAGAAGACACAGAGAAAGAAGTTGCAG
AAGCAGGCCCACGTCGAAAGGAGGCTGCACATAGGGGCAGTGCACAAAGATGGAGTCAAG
TGCTGGAGAAAGACGATCATTACCTCTCCAGAGTCTTTGAATCTCCCTAGAAGAAGCCAT
CCACTCTCCCAGAGTGCTCCAACGGGACTGAACCACATGGGCTGGCCAGAGCACACACCA
GGCACTGCCATGCCTGATGGAGCTCTGGACACAGCTGTCTGCGCTGACGAAGTGGGGAGC
GAGGAGGACCTGTATGATGACCTGCACAGCTCCAGCCACCACTACAGCCACCCTGGAGGG
GGTGGGGAGCAGCTGGCTATCAATGAGCTCATCAGCGATGGCAGTGTGGTCTGCGCTGAA
GCACTCTGGGACCATGTCACCATGGACGACCAGGAGCTGGGCTTCAAAGCTGGGGACGTC
ATCGAAGTGATGGATGCCACCAACAGAGAGTGGTGGTGGGGCCGGGTCGCCGATGGCGAG
GGCTGGTTTCCAGCCAGCTTCGTTCGGCTGAGGGTGAATCAGGACGAGCCCGCGGATGAC
GACGCCCCTCTGGCCGGGAACAGCGGAGCGGAGGACGGCGGGGCGGAGGCGCAGAGCAGC
AAGGACCAGATGCGGACCAACGTCATCAACGAGATCCTCAGCACTGAGCGGGACTACATC
AAGCACCTGCGCGACATCTGCGAGGGCTACGTCCGGCAGTGCCGCAAGCGCGCAGACATG
TTCAGCGAGGAGCAGCTGCGTACCATCTTCGGGAACATCGAGGACATCTACCGCTGCCAG
AAGGCCTTCGTGAAGGCCCTGGAGCAGAGGTTCAACCGCGAGCGCCCACACCTGAGCGAG
CTGGGTGCCTGCTTCCTGGAGCATCAAGCCGACTTCCAGATCTACTCGGAGTACTGCAAT
AACCACCCCAACGCCTGCGTGGAGCTCTCCCGGCTCACCAAGCTCAGCAAGTACGTGTAC
TTCTTCGAGGCCTGCCGGCTGCTGCAGAAGATGATTGACATCTCCCTGGATGGCTTCCTG
CTGACTCCGGTGCAGAAGATCTGCAAGTACCCTCTGCAGCTGGCCGAGCTGCTCAAATAC
ACGCACCCCCAGCACAGGGACTTCAAGGATGTTGAAGCCGCCTTGCATGCCATGAAGAAC
GTGGCCCAGCTCATCAACGAGCGGAAGCGGAGACTTGAGAACATCGACAAGATTGCTCAG
TGGCAGAGCTCCATAGAGGACTGGGAGGGAGAAGATCTCTTGGTCAGGAGCTCAGAACTC
ATCTACTCGGGGGAGCTGACTCGAGTTACACAGCCTCAAGCCAAAAGCCAGCAGCGAATG
TTCTTTCTCTTTGACCACCAGCTCATCTACTGTAAGAAGGACCTGCTCCGCCGCGACGTG
TTGTACTACAAGGGCCGGCTGGACATGGACGGCCTGGAGGTGGTGGACCTGGAGGACGGG
AAGGACAGAGACCTCCATGTGAGCATCAAGAACGCCTTCCGGCTGCACCGTGGCGCCACA
GGGGACAGCCACCTGCTGTGCACCAGGAAGCCCGAGCAGAAGCAGCGCTGGCTCAAGGCC
TTTGCCAGGGAGAGGGAGCAGGTGCAGCTGGACCAGGAGACAGGCTTCTCCATCACTGAA
CTGCAGAGGAAGCAGGCCATGCTGAATGCCAGCAAGCAGCAGGTCACAGGGAAGCCCAAA
GCTGTTGGCCGGCCCTGCTACCTGACGCGCCAGAAGCACCCAGCCCTGCCCAGCAACCGG
CCCCAGCAGCAGGTCCTGGTGCTGGCGGAGCCCAGGCGCAAGCCATCTACCTTCTGGCAC
AGCATCAGCCGGCTGGCACCCTTCCGCAAGTGA
|
| Protein Properties |
|---|
| Number of Residues
| 690 |
| Molecular Weight
| 79065.9 |
| Theoretical pI
| 7.0 |
| Pfam Domain Function
|
|
| Signals
|
|
|
Transmembrane Regions
|
|
| Protein Sequence
|
>Rho guanine nucleotide exchange factor 4
MPWEEPAGEKPSCSHSQKAFHMEPAQKPCFTTDMVTWALLCISAETVRGEAPSQPRGIPH
RSPVSVDDLWLEKTQRKKLQKQAHVERRLHIGAVHKDGVKCWRKTIITSPESLNLPRRSH
PLSQSAPTGLNHMGWPEHTPGTAMPDGALDTAVCADEVGSEEDLYDDLHSSSHHYSHPGG
GGEQLAINELISDGSVVCAEALWDHVTMDDQELGFKAGDVIEVMDATNREWWWGRVADGE
GWFPASFVRLRVNQDEPADDDAPLAGNSGAEDGGAEAQSSKDQMRTNVINEILSTERDYI
KHLRDICEGYVRQCRKRADMFSEEQLRTIFGNIEDIYRCQKAFVKALEQRFNRERPHLSE
LGACFLEHQADFQIYSEYCNNHPNACVELSRLTKLSKYVYFFEACRLLQKMIDISLDGFL
LTPVQKICKYPLQLAELLKYTHPQHRDFKDVEAALHAMKNVAQLINERKRRLENIDKIAQ
WQSSIEDWEGEDLLVRSSELIYSGELTRVTQPQAKSQQRMFFLFDHQLIYCKKDLLRRDV
LYYKGRLDMDGLEVVDLEDGKDRDLHVSIKNAFRLHRGATGDSHLLCTRKPEQKQRWLKA
FAREREQVQLDQETGFSITELQRKQAMLNASKQQVTGKPKAVGRPCYLTRQKHPALPSNR
PQQQVLVLAEPRRKPSTFWHSISRLAPFRK
|
| External Links |
|---|
| GenBank ID Protein
| 15011979 |
| UniProtKB/Swiss-Prot ID
| Q9NR80 |
| UniProtKB/Swiss-Prot Entry Name
| ARHG4_HUMAN |
| PDB IDs
|
Not Available |
| GenBank Gene ID
| NM_015320.2 |
| GeneCard ID
| ARHGEF4 |
| GenAtlas ID
| ARHGEF4 |
| HGNC ID
| HGNC:684 |
| References |
|---|
| General References
| - Nakajima D, Okazaki N, Yamakawa H, Kikuno R, Ohara O, Nagase T: Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones. DNA Res. 2002 Jun 30;9(3):99-106. [PubMed:12168954 ]
- Sjoblom T, Jones S, Wood LD, Parsons DW, Lin J, Barber TD, Mandelker D, Leary RJ, Ptak J, Silliman N, Szabo S, Buckhaults P, Farrell C, Meeh P, Markowitz SD, Willis J, Dawson D, Willson JK, Gazdar AF, Hartigan J, Wu L, Liu C, Parmigiani G, Park BH, Bachman KE, Papadopoulos N, Vogelstein B, Kinzler KW, Velculescu VE: The consensus coding sequences of human breast and colorectal cancers. Science. 2006 Oct 13;314(5797):268-74. Epub 2006 Sep 7. [PubMed:16959974 ]
- Kikuno R, Nagase T, Ishikawa K, Hirosawa M, Miyajima N, Tanaka A, Kotani H, Nomura N, Ohara O: Prediction of the coding sequences of unidentified human genes. XIV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. DNA Res. 1999 Jun 30;6(3):197-205. [PubMed:10470851 ]
- Thiesen S, Kubart S, Ropers HH, Nothwang HG: Isolation of two novel human RhoGEFs, ARHGEF3 and ARHGEF4, in 3p13-21 and 2q22. Biochem Biophys Res Commun. 2000 Jun 24;273(1):364-9. [PubMed:10873612 ]
- Kawasaki Y, Senda T, Ishidate T, Koyama R, Morishita T, Iwayama Y, Higuchi O, Akiyama T: Asef, a link between the tumor suppressor APC and G-protein signaling. Science. 2000 Aug 18;289(5482):1194-7. [PubMed:10947987 ]
- Kawasaki Y, Sato R, Akiyama T: Mutated APC and Asef are involved in the migration of colorectal tumour cells. Nat Cell Biol. 2003 Mar;5(3):211-5. [PubMed:12598901 ]
|
