Hmdb loader
Identification
HMDB Protein ID HMDBP07257
Secondary Accession Numbers
  • 12876
Name Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B
Synonyms
  1. Oligosaccharyl transferase subunit STT3B
  2. STT3-B
  3. Source of immunodominant MHC-associated peptides homolog
Gene Name STT3B
Protein Type Enzyme
Biological Properties
General Function Involved in oligosaccharyl transferase activity
Specific Function Catalytic subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). STT3B is present in a small subset of OST complexes and mediates both cotranslational and post-translational N-glycosylation of target proteins: STT3B-containing complexes are required for efficient cotranslational glycosylation and while they are less competent than STT3A-containing complexes for cotranslational glycosylation, they have the ability to mediate glycosylation of some nascent sites that are not accessible for STT3A. STT3B-containing complexes also act post-translationally and mediate modification of skipped glycosylation sites in unfolded proteins. Plays a role in ER-associated degradation (ERAD) pathway that mediates ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins by mediating N-glycosylation of unfolded proteins, which are then recognized by the ERAD pathway and targeted for degradation. Mediates glycosylation of the disease variant AMYL-TTR 'Asp-38' of TTR at 'Asn-118', leading to its degradation.
Pathways
  • N-Glycan biosynthesis
  • protein glycosylation
  • Protein processing in endoplasmic reticulum
Reactions
Dolichyl diphosphooligosaccharide + [protein]-L-asparagine → Dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine details
Dolichyl diphosphooligosaccharide + Protein asparagine → Dolichyl diphosphate + Glycoprotein with the oligosaccharide chain attached by N-glycosyl linkage to protein L-asparagine details
Protein asparagine + → Dolichyl diphosphate + details
GO Classification
Biological Process
post-translational protein modification
protein N-linked glycosylation via asparagine
glycoprotein catabolic process
co-translational protein modification
ER-associated protein catabolic process
response to unfolded protein
Cellular Component
oligosaccharyltransferase complex
integral to membrane
Component
membrane
cell part
Function
catalytic activity
transferase activity
transferase activity, transferring hexosyl groups
transferase activity, transferring glycosyl groups
oligosaccharyl transferase activity
Molecular Function
dolichyl-diphosphooligosaccharide-protein glycotransferase activity
Process
metabolic process
macromolecule metabolic process
macromolecule modification
protein modification process
protein amino acid glycosylation
Cellular Location
  1. Endoplasmic reticulum membrane
  2. Multi-pass membrane protein
Gene Properties
Chromosome Location 3
Locus 3p23
SNPs STT3B
Gene Sequence
>2481 bp
ATGGCGGAGCCCTCGGCCCCGGAGAGCAAGCACAAGTCGTCCCTCAACTCGTCCCCGTGG
AGTGGCCTCATGGCCCTGGGAAACAGCCGGCACGGCCACCACGGGCCCGGGGCCCAGTGC
GCGCACAAGGCGGCGGGCGGCGCGGCGCCGCCGAAGCCGGCCCCGGCGGGGCTGTCCGGG
GGGCTGTCGCAGCCGGCTGGGTGGCAGTCGCTTCTCTCCTTCACCATCCTCTTCCTGGCC
TGGCTTGCCGGCTTCAGCTCGCGCCTCTTCGCCGTCATCCGCTTCGAAAGCATCATCCAC
GAGTTCGACCCGTGGTTTAACTATAGATCAACACATCATCTTGCATCTCATGGGTTCTAT
GAATTTTTAAATTGGTTTGATGAAAGAGCATGGTATCCACTAGGAAGAATAGTAGGTGGT
ACTGTTTACCCAGGGTTGATGATAACCGCTGGCCTTATTCATTGGATTTTAAATACATTG
AACATAACTGTTCACATAAGAGACGTATGTGTGTTCCTTGCACCAACTTTTAGCGGCCTT
ACATCTATATCTACTTTCCTGCTTACAAGAGAACTTTGGAACCAAGGAGCAGGACTTTTA
GCTGCTTGTTTTATTGCTATTGTACCAGGCTACATATCTCGGTCAGTAGCTGGATCCTTT
GATAATGAAGGCATTGCTATTTTTGCACTTCAGTTCACATACTATTTATGGGTAAAATCT
GTAAAAACTGGGTCAGTTTTTTGGACAATGTGCTGCTGCTTATCCTATTTCTATATGGTC
TCTGCTTGGGGTGGTTATGTATTTATCATCAATCTTATTCCACTGCATGTATTTGTGTTG
TTACTGATGCAGAGATACAGCAAAAGAGTCTACATAGCATATAGCACTTTCTACATTGTG
GGTTTAATATTATCAATGCAGATACCTTTTGTGGGATTCCAGCCAATCAGAACAAGTGAA
CACATGGCAGCTGCAGGTGTCTTTGCATTGCTGCAAGCTTATGCTTTCTTGCAGTATCTG
AGAGACCGATTAACAAAACAAGAGTTCCAGACCCTTTTCTTTTTGGGTGTATCACTAGCT
GCAGGTGCTGTGTTCCTTAGTGTCATCTATTTGACTTATACAGGTTACATTGCACCATGG
AGTGGCAGGTTTTATTCATTGTGGGATACTGGGTATGCAAAAATACACATTCCAATTATT
GCATCAGTGTCTGAGCATCAACCTACGACTTGGGTGTCTTTCTTCTTTGATCTACATATT
CTTGTATGTACCTTCCCAGCAGGCCTTTGGTTCTGCATCAAAAATATCAACGATGAAAGA
GTATTTGTTGCTCTATATGCAATCAGTGCTGTCTACTTTGCTGGAGTGATGGTGCGACTG
ATGTTGACTTTGACTCCAGTCGTGTGTATGCTGTCTGCAATTGCCTTTTCAAATGTTTTT
GAGCACTATTTGGGGGATGACATGAAAAGGGAAAATCCACCTGTGGAGGACAGCAGTGAT
GAGGATGACAAAAGAAACCAAGGAAATTTGTATGATAAGGCAGGTAAAGTGAGGAAACAT
GCAACTGAACAGGAAAAAACTGAAGAGGGATTAGGCCCTAATATAAAAAGCATTGTCACC
ATGTTGATGCTGATGCTATTGATGATGTTTGCTGTCCACTGTACCTGGGTCACAAGCAAT
GCCTACTCTAGTCCAAGTGTAGTCCTGGCCTCATACAATCATGATGGCACCAGGAATATC
TTAGATGATTTTAGAGAAGCTTACTTTTGGCTAAGGCAAAATACAGATGAACATGCACGA
GTAATGTCTTGGTGGGATTATGGCTATCAGATAGCTGGAATGGCTAATAGAACTACGTTG
GTGGATAATAACACCTGGAATAACAGCCACATAGCACTGGTGGGAAAAGCTATGTCTTCT
AATGAAACAGCAGCCTATAAAATCATGAGGACTCTAGATGTAGATTATGTTTTGGTTATT
TTTGGAGGGGTTATTGGCTATTCTGGTGATGATATCAACAAATTTCTCTGGATGGTTAGG
ATAGCTGAAGGAGAACATCCCAAAGACATTCGGGAAAGTGACTATTTTACCCCACAGGGA
GAATTCCGTGTAGACAAAGCAGGATCCCCTACTTTGTTGAATTGCCTTATGTATAAAATG
TCATACTACAGATTTGGAGAAATGCAGCTGGATTTTCGTACACCCCCAGGTTTTGACCGA
ACACGTAATGCTGAGATTGGAAATAAGGACATTAAATTCAAACATTTGGAAGAAGCCTTT
ACATCAGAACACTGGCTTGTTAGGATATATAAAGTAAAAGCACCTGATAACAGGGAGACA
TTAGATCACAAACCTCGAGTCACCAACATTTTCCCAAAACAGAAGTATTTGTCAAAGAAG
ACTACCAAAAGGAAGCGTGGCTACATTAAAAATAAGCTGGTTTTTAAGAAAGGCAAGAAA
ATATCTAAGAAGACTGTTTAA
Protein Properties
Number of Residues 826
Molecular Weight 93673.495
Theoretical pI 8.911
Pfam Domain Function
Signals Not Available
Transmembrane Regions Not Available
Protein Sequence
>Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B
MAEPSAPESKHKSSLNSSPWSGLMALGNSRHGHHGPGAQCAHKAAGGAAPPKPAPAGLSG
GLSQPAGWQSLLSFTILFLAWLAGFSSRLFAVIRFESIIHEFDPWFNYRSTHHLASHGFY
EFLNWFDERAWYPLGRIVGGTVYPGLMITAGLIHWILNTLNITVHIRDVCVFLAPTFSGL
TSISTFLLTRELWNQGAGLLAACFIAIVPGYISRSVAGSFDNEGIAIFALQFTYYLWVKS
VKTGSVFWTMCCCLSYFYMVSAWGGYVFIINLIPLHVFVLLLMQRYSKRVYIAYSTFYIV
GLILSMQIPFVGFQPIRTSEHMAAAGVFALLQAYAFLQYLRDRLTKQEFQTLFFLGVSLA
AGAVFLSVIYLTYTGYIAPWSGRFYSLWDTGYAKIHIPIIASVSEHQPTTWVSFFFDLHI
LVCTFPAGLWFCIKNINDERVFVALYAISAVYFAGVMVRLMLTLTPVVCMLSAIAFSNVF
EHYLGDDMKRENPPVEDSSDEDDKRNQGNLYDKAGKVRKHATEQEKTEEGLGPNIKSIVT
MLMLMLLMMFAVHCTWVTSNAYSSPSVVLASYNHDGTRNILDDFREAYFWLRQNTDEHAR
VMSWWDYGYQIAGMANRTTLVDNNTWNNSHIALVGKAMSSNETAAYKIMRTLDVDYVLVI
FGGVIGYSGDDINKFLWMVRIAEGEHPKDIRESDYFTPQGEFRVDKAGSPTLLNCLMYKM
SYYRFGEMQLDFRTPPGFDRTRNAEIGNKDIKFKHLEEAFTSEHWLVRIYKVKAPDNRET
LDHKPRVTNIFPKQKYLSKKTTKRKRGYIKNKLVFKKGKKISKKTV
GenBank ID Protein 30578410
UniProtKB/Swiss-Prot ID Q8TCJ2
UniProtKB/Swiss-Prot Entry Name STT3B_HUMAN
PDB IDs Not Available
GenBank Gene ID NM_178862.1
GeneCard ID STT3B
GenAtlas ID STT3B
HGNC ID HGNC:30611
References
General References
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