| Identification |
|---|
| HMDB Protein ID
| HMDBP05922 |
| Secondary Accession Numbers
| |
| Name
| Alpha-(1,6)-fucosyltransferase |
| Synonyms
|
- Fucosyltransferase 8
- GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha1,6-fucosyltransferase
- GDP-fucose--glycoprotein fucosyltransferase
- Glycoprotein 6-alpha-L-fucosyltransferase
- alpha1-6FucT
|
| Gene Name
| FUT8 |
| Protein Type
| Enzyme |
| Biological Properties |
|---|
| General Function
| Involved in glycoprotein 6-alpha-L-fucosyltransferase activity |
| Specific Function
| Catalyzes the addition of fucose in alpha 1-6 linkage to the first GlcNAc residue, next to the peptide chains in N-glycans.
|
| Pathways
|
- Glycosaminoglycan biosynthesis - keratan sulfate
- N-Glycan biosynthesis
- protein glycosylation
- Transcriptional misregulation in cancer
|
| Reactions
|
| GDP-L-fucose + N(4)-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6))-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl)asparagine → Guanosine diphosphate + N(4)-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6))-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-(alpha-L-fucosyl-(1->6))-N-acetyl-beta-D-glucosaminyl)asparagine |
details
|
| GDP-L-fucose + → GDP + |
details
|
| GDP-L-fucose + → GDP + |
details
|
|
| GO Classification
|
| Biological Process |
| oligosaccharide biosynthetic process |
| L-fucose catabolic process |
| cell migration |
| integrin-mediated signaling pathway |
| N-glycan processing |
| protein glycosylation in Golgi |
| receptor metabolic process |
| in utero embryonic development |
| respiratory gaseous exchange |
| post-translational protein modification |
| protein N-linked glycosylation via asparagine |
| transforming growth factor beta receptor signaling pathway |
| Cellular Component |
| Golgi cisterna membrane |
| integral to membrane |
| Golgi membrane |
| Component |
| golgi membrane |
| membrane |
| cell part |
| membrane part |
| organelle membrane |
| intrinsic to membrane |
| integral to membrane |
| golgi cisterna membrane |
| Function |
| catalytic activity |
| transferase activity |
| transferase activity, transferring hexosyl groups |
| transferase activity, transferring glycosyl groups |
| fucosyltransferase activity |
| alpha(1,6)-fucosyltransferase activity |
| glycoprotein 6-alpha-l-fucosyltransferase activity |
| Molecular Function |
| glycoprotein 6-alpha-L-fucosyltransferase activity |
| Process |
| metabolic process |
| macromolecule metabolic process |
| macromolecule modification |
| protein modification process |
| protein amino acid glycosylation |
| protein amino acid glycosylation in golgi |
|
| Cellular Location
|
- Single-pass type II membrane protein
- Golgi apparatus
- Golgi stack membrane
|
| Gene Properties |
|---|
| Chromosome Location
| 14 |
| Locus
| 14q24.3 |
| SNPs
| FUT8 |
| Gene Sequence
|
>1728 bp
ATGCGGCCATGGACTGGTTCCTGGCGTTGGATTATGCTCATTCTTTTTGCCTGGGGGACC
TTGCTGTTTTATATAGGTGGTCACTTGGTACGAGATAATGACCATCCTGATCACTCTAGC
CGAGAACTGTCCAAGATTCTGGCAAAGCTTGAACGCTTAAAACAACAGAATGAAGACTTG
AGGCGAATGGCCGAATCTCTCCGGATACCAGAAGGCCCTATTGATCAGGGGCCAGCTATA
GGAAGAGTACGCGTTTTAGAAGAGCAGCTTGTTAAGGCCAAAGAACAGATTGAAAATTAC
AAGAAACAGACCAGAAATGGTCTGGGGAAGGATCATGAAATCCTGAGGAGGAGGATTGAA
AATGGAGCTAAAGAGCTCTGGTTTTTCCTACAGAGTGAATTGAAGAAATTAAAGAACTTA
GAAGGAAATGAACTCCAAAGACATGCAGATGAATTTCTTTTGGATTTAGGACATCATGAA
AGGTCTATAATGACGGATCTATACTACCTCAGTCAGACAGATGGAGCAGGTGATTGGCGG
GAAAAAGAGGCCAAAGATCTGACAGAACTGGTTCAGCGGAGAATAACATATCTTCAGAAT
CCCAAGGACTGCAGCAAAGCCAAAAAGCTGGTGTGTAATATCAACAAAGGCTGTGGCTAT
GGCTGTCAGCTCCATCATGTGGTCTACTGCTTCATGATTGCATATGGCACCCAGCGAACA
CTCATCTTGGAATCTCAGAATTGGCGCTATGCTACTGGTGGATGGGAGACTGTATTTAGG
CCTGTAAGTGAGACATGCACAGACAGATCTGGCATCTCCACTGGACACTGGTCAGGTGAA
GTGAAGGACAAAAATGTTCAAGTGGTCGAGCTTCCCATTGTAGACAGTCTTCATCCCCGT
CCTCCATATTTACCCTTGGCTGTACCAGAAGACCTCGCAGATCGACTTGTACGAGTGCAT
GGTGACCCTGCAGTGTGGTGGGTGTCTCAGTTTGTCAAATACTTGATCCGCCCACAGCCT
TGGCTAGAAAAAGAAATAGAAGAAGCCACCAAGAAGCTTGGCTTCAAACATCCAGTTATT
GGAGTCCATGTCAGACGCACAGACAAAGTGGGAACAGAAGCTGCCTTCCATCCCATTGAA
GAGTACATGGTGCATGTTGAAGAACATTTTCAGCTTCTTGCACGCAGAATGCAAGTGGAC
AAAAAAAGAGTGTATTTGGCCACAGATGACCCTTCTTTATTAAAGGAGGCAAAAACAAAG
TACCCCAATTATGAATTTATTAGTGATAACTCTATTTCCTGGTCAGCTGGACTGCACAAT
CGATACACAGAAAATTCACTTCGTGGAGTGATCCTGGATATACATTTTCTCTCTCAGGCA
GACTTCCTAGTGTGTACTTTTTCATCCCAGGTCTGTCGAGTTGCTTATGAAATTATGCAA
ACACTACATCCTGATGCCTCTGCAAACTTCCATTCTTTAGATGACATCTACTATTTTGGG
GGCCAGAATGCCCACAATCAAATTGCCATTTATGCTCACCAACCCCGAACTGCAGATGAA
ATTCCCATGGAACCTGGAGATATCATTGGTGTGGCTGGAAATCATTGGGATGGCTATTCT
AAAGGTGTCAACAGGAAATTGGGAAGGACGGGCCTATATCCCTCCTACAAAGTTCGAGAG
AAGATAGAAACGGTCAAGTACCCCACATATCCTGAGGCTGAGAAATAA
|
| Protein Properties |
|---|
| Number of Residues
| 575 |
| Molecular Weight
| 66515.32 |
| Theoretical pI
| 7.657 |
| Pfam Domain Function
|
Not Available |
| Signals
|
Not Available
|
|
Transmembrane Regions
|
Not Available
|
| Protein Sequence
|
>Alpha-(1,6)-fucosyltransferase
MRPWTGSWRWIMLILFAWGTLLFYIGGHLVRDNDHPDHSSRELSKILAKLERLKQQNEDL
RRMAESLRIPEGPIDQGPAIGRVRVLEEQLVKAKEQIENYKKQTRNGLGKDHEILRRRIE
NGAKELWFFLQSELKKLKNLEGNELQRHADEFLLDLGHHERSIMTDLYYLSQTDGAGDWR
EKEAKDLTELVQRRITYLQNPKDCSKAKKLVCNINKGCGYGCQLHHVVYCFMIAYGTQRT
LILESQNWRYATGGWETVFRPVSETCTDRSGISTGHWSGEVKDKNVQVVELPIVDSLHPR
PPYLPLAVPEDLADRLVRVHGDPAVWWVSQFVKYLIRPQPWLEKEIEEATKKLGFKHPVI
GVHVRRTDKVGTEAAFHPIEEYMVHVEEHFQLLARRMQVDKKRVYLATDDPSLLKEAKTK
YPNYEFISDNSISWSAGLHNRYTENSLRGVILDIHFLSQADFLVCTFSSQVCRVAYEIMQ
TLHPDASANFHSLDDIYYFGGQNAHNQIAIYAHQPRTADEIPMEPGDIIGVAGNHWDGYS
KGVNRKLGRTGLYPSYKVREKIETVKYPTYPEAEK
|
| External Links |
|---|
| GenBank ID Protein
| 30410724 |
| UniProtKB/Swiss-Prot ID
| Q9BYC5 |
| UniProtKB/Swiss-Prot Entry Name
| FUT8_HUMAN |
| PDB IDs
|
|
| GenBank Gene ID
| NM_178154.1 |
| GeneCard ID
| FUT8 |
| GenAtlas ID
| FUT8 |
| HGNC ID
| HGNC:4019 |
| References |
|---|
| General References
| - Yanagidani S, Uozumi N, Ihara Y, Miyoshi E, Yamaguchi N, Taniguchi N: Purification and cDNA cloning of GDP-L-Fuc:N-acetyl-beta-D-glucosaminide:alpha1-6 fucosyltransferase (alpha1-6 FucT) from human gastric cancer MKN45 cells. J Biochem. 1997 Mar;121(3):626-32. [PubMed:9133635 ]
- Yamaguchi Y, Ikeda Y, Takahashi T, Ihara H, Tanaka T, Sasho C, Uozumi N, Yanagidani S, Inoue S, Fujii J, Taniguchi N: Genomic structure and promoter analysis of the human alpha1, 6-fucosyltransferase gene (FUT8). Glycobiology. 2000 Jun;10(6):637-43. [PubMed:10814706 ]
- Takahashi T, Ikeda Y, Tateishi A, Yamaguchi Y, Ishikawa M, Taniguchi N: A sequence motif involved in the donor substrate binding by alpha1,6-fucosyltransferase: the role of the conserved arginine residues. Glycobiology. 2000 May;10(5):503-10. [PubMed:10764839 ]
|
